In-frame deletion of Escherichia coli essential genes in complex regulon
A conditional knockout-rescue system was developed to construct an in-frame deletion strain of Escherichia coli essential genes.The target was flanked 2005 honda accord intake manifold with marker genes and FRT (FLP recognition target) sites, and a plasmid containing arabinose-induced FLP recombinase was transformed.After arabinose induction, cells